MSH2 Mouse Monoclonal Antibody [Clone ID: 1B3=3A2B8C]

Specifications

Product Data
Clone Name	1B3=3A2B8C
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA: 1/10000.  Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000.  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human MSH2 expressed in E. Coli.
Specificity	Recognizes MSH2
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	105 kDa
Gene Name	mutS homolog 2
Database Link	Entrez Gene 4436 Human UniProt ID P43246
Background	MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
Synonyms	DNA mismatch repair protein Msh2, MutS protein homolog 2
Reference Data

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