GFP Mouse Monoclonal Antibody [Clone ID: 4B10B2]

Specifications

Product Data
Clone Name	4B10B2
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Bloting: 1/500 - 1/2000. Flow Cytometry: 1/200 - 1/400.  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	GFP
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of GFP expressed in E. Coli.
Specificity	Recognizes GFP (Green Fluorescence Protein).
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.03% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	27 kDa
Database Link	UniProt ID P42212
Background	GFP (Green Fluorescence Protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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