Horseradish Peroxidase / HRP Mouse Monoclonal Antibody [Clone ID: 3A5C6]

Specifications

Product Data
Clone Name	3A5C6
Applications	WB
Recommend Dilution	ELISA: 1/10000. Western Bloting: 1/500 - 1/2000.
Reactivity	Horseradish Peroxidase
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of HRP expressed in E. Coli.
Specificity	Recognizes Horseradish Peroxidase / HRP
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.
Reference Data

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