Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody [Clone ID: 1D11C6E6]

Specifications

Product Data
Clone Name	1D11C6E6
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blotting: 1/500 - 1/2000. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of CRYAB (aa1-175) expressed in E. Coli.
Specificity	This antibody recognizes Alpha Crystallin B Chain (CRYAB).
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	crystallin alpha B
Database Link	Entrez Gene 1410 Human UniProt ID P02511
Background	Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Synonyms	Alpha(B)-crystallin, CRYAB, Heat shock protein beta-5, Renal carcinoma antigen NY-REN-27, HspB5
Reference Data

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