Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: 4F2E9]

Specifications

Product Data
Clone Name	4F2E9
Applications	IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.
Specificity	Recognizes Vimentin.
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	vimentin
Database Link	Entrez Gene 7431 Human UniProt ID P08670
Background	Vimentin, also know as VIM is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Synonyms	VIM
Reference Data

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