CDC27 Mouse Monoclonal Antibody [Clone ID: 5C12]

Specifications

Product Data
Clone Name	5C12
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Immunohistochemistry on Paraffin: 1/200 - 1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human CDC27 expressed in E. Coli.
Specificity	Recognizes CDC27
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	91 kDa
Gene Name	cell division cycle 27
Database Link	Entrez Gene 996 Human UniProt ID P30260
Background	Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Synonyms	CDC27Hs, H-NUC, D0S1430E, D17S978E
Reference Data

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