Cytochrome P450 3A4 (CYP3A4) Mouse Monoclonal Antibody [Clone ID: 3H8]

Specifications

Product Data
Clone Name	3H8
Applications	ELISA, FC, IF, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500-1/2000. Flow Cytometry: 1/200-1/400. Immunofluorescence: 1/200-1/1000. Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human CYP3A4 expressed in E. Coli
Specificity	This antibody recognizes Human CYP3A4.
Formulation	PBS with 0.05% Sodium Azide as preservative and 0.5% protein stabilizer State: Purified State: Liquid purified antibody
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	57.3 kDa
Gene Name	cytochrome P450 family 3 subfamily A member 4
Database Link	Entrez Gene 1576 Human UniProt ID P08684
Background	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Synonyms	CYP3A3, Cytochrome P450 3A4, CYPIIIA4, Nifedipine oxidase, Cytochrome P450 3A3, CYPIIIA3, Cytochrome P450 HLp, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Albendazole monooxygenase, Albendazole sulfoxidase
Note	Protocol: Tissue Array Results Hepatic Carcinoma: Position: A5/6 and B5/6, Localization: Cytoplasm, Result: + Liver: Position: C5/6, Localization: Cytoplasm, Result: + Renal Cell Carcinoma: Position: D5/6, Result: - Kidney: Position: E5/6, Result: - Pancreas: Position: F5/6, Result: - Prostate Cancer: Position: G5/6, Result: - Lymphoid tissue: Position: H5/6, Result: - Lung Cancer: Position: A7/8 and B7/8, Result: - Lung: Position: C7/8, Result: - Brain: Position: D7/8, Localization: Nucleus and Cytoplasm, Result: + Brain: Position: E7/8, Localization: Cytoplasm, Result: + Medulla oblongata: Position: F7/8, Localization: Cytoplasm, Result: + Cerebellar: Position: G7/8, Localization: Cytoplasm, Result: + Esophageal Cancer: Position: A1/2 and B1/2, Result: - Esophagus: Position: Position: C1/2 and D1/2, Result: - Stomach Cancer: Position: E1/2 and F1/2, Result: - Stomach: Position: G1/2 and H1/2, Result: - Colon Cancer: Position: A3/4 and B3/4, Result: - Colon: Position: C3/4, Result: - Duodenum: Position: C3/4, Result: - Rectal Cancer: Position: E3/4 and F3/4, Result: - Rectum: Position: G3/4, Result: - Testis: Position: H3/4, Result: -
Reference Data

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