Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]

Specifications

Product Data
Clone Name	53-6.7
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: < / = 2 µg/10e6 cells. (Ref.8,9) Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref. 10) Immunoprecipitation. (Ref.8) In vivo and in vitro depletion of CD8a+ cells. (Ref. 1,4,5) Fractionation of CD8a+ T cells. (Ref.10)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8 antigen, alpha chain
Database Link	Entrez Gene 12525 Mouse UniProt ID P01731
Background	In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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