Ctla4 Hamster Monoclonal Antibody [Clone ID: 9H10]

Specifications

Product Data
Clone Name	9H10
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry: < / = 3 µg/10e6 cells. Suggest use of these reagents is in a final volume 100µl. Immunoprecipitation. In vitro and in vivo blocking of CTLA-4 action by soluble antibody. Promotion of in vitro CTLA-4 signalling by cross-linked antibody. See Ref.1 for more details.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	Heat-killed Staphylococcus A bacteria coated with mouse CTLA-4/human IgG1 fusion protein. (Ref.1)
Specificity	This Monoclonal antibody is specific to CD152/CTLA-4 costimulatory receptor (Mr 33-37 kDa).
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cytotoxic T-lymphocyte-associated protein 4
Database Link	Entrez Gene 12477 Mouse UniProt ID P09793
Background	CD152, also known as CTLA-4, is homologous to the costimulatory molecule CD28. (Ref.1,2) Like CD28, the CD152 molecule binds the family of B7 counter-receptors (CD80/B7-1 and CD86/B7-2) on antigen-presenting cells. (Ref.3) CTLA-4 is not expressed on resting T lymphocytes but is induced 2-3 days following T-cell stimulation via the T cell receptor (TCR) complex. (Ref.1,3,4) Whereas CD28 provides a positive costimulatory signal, CD152/CTLA-4 is a negative regulator of T-cell activation, possibly by inhibiting tyrosine kinase signalling through the TCR through its association with tyrosine phosphatases such as SHP-2. (Ref.1,5,6)
Synonyms	CTLA-4
Reference Data

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