CD45 / LCA (CD45.1 alloantigen) Mouse Monoclonal Antibody [Clone ID: A20]

Specifications

Product Data
Clone Name	A20
Applications	FC, FN, IF, IP
Recommend Dilution	Flow Cytometry and Fluorescence Microscopy. (Ref.3,4) Immunoprecipitation. Markers for origin of donor and host cells. (Ref.3,4)
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Specificity	The monoclonal antibody A20 recognizes all mouse leukocytes expressing the CD45.1 allotype.
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Background	The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. (Ref.1,2) This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. (Ref.1,2) The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products AM08044, AM08045, AM08046) and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. (Ref.3,4)
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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