Klrg1 Hamster Monoclonal Antibody [Clone ID: 2F1]

Specifications

Product Data
Clone Name	2F1
Applications	FC, IP
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1,5) Immunoprecipitation. (Ref.1,5)
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	Activated NK (A-LAK) cells from B6 (H-2b) mice. (Ref.1)
Specificity	This antibody reacts with Killer Cell Lectin-like Receptor G1 (KLRG1 formerly known as mouse MAFA or 2F1-Ag). It stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). (Ref.1,5)
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	killer cell lectin-like receptor subfamily G, member 1
Database Link	Entrez Gene 50928 Mouse UniProt ID O88713
Background	Killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1 Ag), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. (Ref.1,2) These ITIMs interact with the SH2 domains of protein phosphatases such as SHP1. (Ref.3,4) Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors. (Ref.1)
Synonyms	KLRG1, CLEC15A, MAFA, MAFAL, Mast cell function-associated antigen, MAFA-like receptor, ITIM-containing receptor MAFA-L
Reference Data

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