MHC Class I H2 Kd/Dd Mouse Monoclonal Antibody [Clone ID: 34-1-2S]

CAT#: AM08081FC-S

MHC Class I H2 Kd/Dd mouse monoclonal antibody, clone 34-1-2S, FITC

Conjugation: Unconjugated Biotin FITC PE



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CNY 3,710.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 34-1-2S
Applications FC
Recommend Dilution Flow Cytometry.
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Immunogen BDF splenocytes
Donor: C3H spleen
Fusion Partner: Sp2/0-Ag14
Specificity Anti H-2KdDd mAb reacts with both H-2Kd and H-2Dd products. The antibody also cross reacts with Kbsrpq. This K-D cross reaction indicates the presence of shared specificities between the two separate H-2 regions.
Formulation PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein
Label: FITC
State: Liquid purified Ig fraction
Label: Fluorescein isothiocyanate isomer 1
Concentration lot specific
Purification Affinity chromatography on Protein G
Conjugation FITC
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M; cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
4. To each tube, add 0.5 - 0.2 μg of this antibody per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 μl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls).
Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per test
Antibody Concentration Used: 0.5 μg/10e6 cells
Isotypic Control: FITC Mouse IgG2a
Percentage of cells stained above control:
Thymus 91.9%
Spleen 95.1%
Lymph Node 100%
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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