CD5 Mouse Monoclonal Antibody [Clone ID: f43]

Specifications

Product Data
Clone Name	f43
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry. (Ref.1-2) Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections only). Immunoprecipitation. (Ref.1)
Reactivity	Feline
Host	Mouse
Clonality	Monoclonal
Specificity	Specific to Feline CD5. (Mr. 67kDa) This antibody is useful as a pan T Cell Marker. (Ref.1)
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms	LEU1, LEU-1, Ly-1, Lyt-1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5
Reference Data

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