CD3 Mouse Monoclonal Antibody [Clone ID: CT-3]

Specifications

Product Data
Clone Name	CT-3
Applications	FC
Recommend Dilution	Flow Cytometry: ≤ 1 µg/106 cells. (Ref.1)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Specificity	Specific to Chicken CD3. This antibody recognizes a complex of at least three polypeptides of Mr 20-, 19-, and 17-kDa (two of which are N-glycosylated) on Chicken T cells. It also coprecipitates a polypeptide of 90-kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50- and 40-kDa. (Ref.1)
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified Ig fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q98910
Background	T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data

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