LOC396098 (Bu1a + Bu1b) Mouse Monoclonal Antibody [Clone ID: AV20]

Specifications

Product Data
Clone Name	AV20
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.2) Immunoprecipitation. (Ref.2) Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections).
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens. (Ref.1)
Specificity	This antibody recognizes a monomorphic determinant on the Bu-1 B cell associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. It also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. (Ref.1-2) No Cross reactivity is observed with Turkey cells when analyzed by Immunofluorescent staining and Flow Cytometry.
Formulation	100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	B6.1
Database Link	Entrez Gene 396098 Chicken UniProt ID Q90746
Background	The Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance.
Synonyms	B-Cell Marker
Reference Data

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