LAMP2 Mouse Monoclonal Antibody [Clone ID: H4B4]

Specifications

Product Data
Clone Name	H4B4
Applications	FC, IHC, WB
Recommend Dilution	Western Blot: 2 µg/ml. Flow Cytometry: ≤ 1 µg/106 cells. Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). Immunohistochemistry on Paraffin Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human adherent peripheral blood cells
Specificity	This antibody recognizes Human CD107b/LAMP-2. Other species not tested.
Formulation	100 mM Borate Buffered Saline, pH 8.2 without preservatives. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	lysosomal associated membrane protein 2
Database Link	Entrez Gene 3920 Human UniProt ID P13473
Background	CD107b, also known as lysosomal-associated membrane protein 2 (LAMP-2), is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107b is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-2 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium. (Ref.1-7)
Synonyms	LAMP-2, LAMP-2C, LAMPB
Reference Data

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