DYKDDDDK Epitope Tag Mouse Monoclonal Antibody [Clone ID: 29E4.G7.H11]

Specifications

Product Data
Clone Name	29E4.G7.H11
Applications	ELISA, FC, IP, WB
Recommend Dilution	This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy-termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/20,000. Flow Cytometry: 1/2,000-1/10,000.
Host	Mouse
Clonality	Monoclonal
Immunogen	Produced in Mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
Specificity	Carboxy and amino terminal linked FLAG™ tagged recombinant proteins. This antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified IgG fraction.
Concentration	lot specific
Purification	Protein A Chromatography followed by extensive dialysis against the buffer.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Synonyms	FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag
Reference Data

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