MTBP (667-812) Mouse Monoclonal Antibody [Clone ID: PHL-1]

Specifications

Product Data
Clone Name	PHL-1
Applications	ELISA, WB
Recommend Dilution	ELISA: 1:2,000 - 1:20,000. Western blot: 1:200 - 1:2,000.
Reactivity	Chimpanzee, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein
Specificity	This antibody is directed against human MTBP protein.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide State: Ascites State: Liquid sterile filtered Ig fraction
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C for longer. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Gene Name	MDM2 binding protein
Database Link	Entrez Gene 27085 Human UniProt ID Q96DY7
Background	The MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor. Studies also indicate that MDM2 regulates cell proliferation via p53 independent mechanisms. MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1). MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2). The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).
Synonyms	Mdm2-binding protein, hMTBP, Mdm2
Note	Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence.
Reference Data

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