FKBP52 (FKBP4) Mouse Monoclonal Antibody [Clone ID: AT4D3]

Specifications

Product Data
Clone Name	AT4D3
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot (1/1,000-1/2,000). Cell lysates of HeLa (40 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FkBP4 (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Immunohistochemistry on Paraffin Embedded Tissue (1/50-1/100). Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-Human FKBP4 (1/50) for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human FKBP4 (1-459 aa) purified from E. coli
Specificity	The antibody recognizes Human FKBP4. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	FK506 binding protein 4
Database Link	Entrez Gene 2288 Human UniProt ID Q02790
Background	FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes.
Synonyms	FK506-binding protein 4, FKBP52 protein, FKBP59, p59 protein
Reference Data
Protein Families	Druggable Genome

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