Desmoplakin (DSP) (C-term) Mouse Monoclonal Antibody [Clone ID: DPI/II 236.23.1]

Specifications

Product Data
Clone Name	DPI/II 236.23.1
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Sections (Microwave retrieval method required). Working Dilution: Ready to Use for Immunohistochemistry. Incubation Time: 1 hour at RT.
Reactivity	Bovine, Human, Mouse, Xenopus
Host	Mouse
Clonality	Monoclonal
Immunogen	C-terminal polypeptide of recombinant Human Desmoplakin (starting at amino acid position 1980)
Specificity	The antibody shows distinct punctate membrane staining of different epithelia. Desmosomes of the myocardium are also positive. Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma. Tested Reactivities on Cultured Cell Lines: Several human carcinoma cell lines: MCF-7, A-431, HaCaT, CaCo2; bovine cells: MDBK, BMGE. Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000).
Formulation	State: Supernatant State: Liquid Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	desmoplakin
Database Link	Entrez Gene 1832 Human UniProt ID P15924
Background	Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Synonyms	DP, DSP, 250/210 kDa paraneoplastic pemphigus antigen
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC)

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