Desmoglein 1 (DSG1) Mouse Monoclonal Antibody [Clone ID: Dsg1-P23]

Specifications

Product Data
Clone Name	Dsg1-P23
Applications	IHC, WB
Recommend Dilution	Immunoblotting (Western).  Immunohistochemistrry on Frozen Sections: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended (See Ref.1) Immunohistochemistry on Paraffin Embedded Sections (After Microwave treatment): For better staining on paraffin sections preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media is recommended. Recommneded Dilutions: 1/1000 for 1h at RT for Immunohistochemistry (Dilute immediately before use in PBS).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human polypeptide Desmoglein 1 (extracellular part EII, EIII and EIV)
Specificity	In Immunoblots of cytoskeletal preparations from Human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1. In immunolocalisation studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera.  Cross reaction with Desmoglein 2 and 3 was not detected.
Formulation	State: Supernatant State: Liquid Hybridoma Culture Supernatant (Concentrate) Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	desmoglein 1
Database Link	Entrez Gene 1828 Human UniProt ID Q02413
Background	Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus. Desmoglein 1 is a 165kD glycoprotein that is a core component of the desmosome cell-cell junction between epithelial cells. Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.
Synonyms	Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DSG1, CDHF4, DG1, DGI
Reference Data
Protein Families	Druggable Genome

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