EGF Mouse Monoclonal Antibody [Clone ID: S-146]

Specifications

Product Data
Clone Name	S-146
Applications	ELISA, IF, WB
Recommend Dilution	ELISA: The antibody can be used in Sandwich ELISA for EGF detection. The Matched antibody (Capture) is Clone S-134 Cat.-No DM1012. Western blot: The antibody recognized recombinant EGF. Immunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human EGF, 6kDa, 53 amino acid residues
Specificity	Reactive with natural and recombinant Human EGF. Does not cross react with other Human cytokines tested such as IL-1beta, IL-8, MCAF, TGF-beta and SAA
Formulation	0.01M PBS, pH 7.2 without preservatives State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	epidermal growth factor
Database Link	Entrez Gene 1950 Human UniProt ID P01133
Background	Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
Synonyms	Urogastrone, Epidermal growth factor, URG, HOMG4
Reference Data
Protein Families	Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
Protein Pathways	Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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