pan Cytokeratin Mouse Monoclonal Antibody [Clone ID: AE1/AE3]

Specifications

Product Data
Clone Name	AE1/AE3
Applications	FC, IF
Recommend Dilution	Immunofluorescence: 10-20 µg /ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4°C. Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000) (Not tested in our lab).
Reactivity	Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human epidermal keratin.
Specificity	AE 1/AE3 represents an excellent marker for distinguishing carcinomas from non-epithelial tumors; reacts with all epithelium-derived tumors and their neoplasms. Decorates the majority of type I and type II keratins (formerly also designated cytokeratins).  This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. This antibody AE 1/AE 3 recognizes Low Molecular Weight Cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40) of the acidic family and CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58) CK6 (56) and CK8 (52.5). Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 Label: FITC State: Purified State: Liquid purified IgG fraction Stabilizer: 1% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).
Synonyms	pan Keratin, Cytokeratin pan-reactive
Reference Data

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