basic Cytokeratin Mouse Monoclonal Antibody [Clone ID: AE3]

Specifications

Product Data
Clone Name	AE3
Applications	IF, IHC
Recommend Dilution	Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears) For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes). Immunofluorescence. Recommended Positive Control: Human skin, Lung Ca.
Reactivity	Bovine, Chicken, Human, Monkey, Mouse, Porcine, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human epidermal keratin.
Specificity	This antibody reacts with CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58) CK6(56) and CK8 (52.5). Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein A Chromatography.
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).
Reference Data

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