TMEM16A (ANO1) Mouse Monoclonal Antibody [Clone ID: DOG1.1]

Specifications

Product Data
Clone Name	DOG1.1
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100. Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Breast carcinomas.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptides FLJ34272 specific to Human Gastrointestinal Stromal Tumor (GIST)
Specificity	DOG1-1 Monoclonal antibody yielded positive staining in 370 of 425 (87%) GIST, whereas CD117 was positive in 317 of 428 (74%) GIST. For special GISTs, DOG1 immunoreactivity was detected in 23/29 (79%) GISTs with mutations, while only 9% (3/32) stained for CD117; in 10/28 (36%) KIT-negative GISTs; in 16 (100%) NF1-associated GISTs; and in 9/11 (82%) pediatric GISTs. Only 1 of 326 (0.3%) leiomyosarcomas and 1 of 39 (2.5%) synovial sarcomas among the 935 soft tissue tumors examined showed positive immunostaining for DOG1.1. In addition, DOG1.1 immunoreactivity was seen in fewer cases of carcinoma, melanoma, and seminoma as compared with KIT. Therefore, DOG1.1 is a sensitive and specific immunohistochemical marker for GIST, comparable with KIT, with the additional benefit of detecting KIT-negative GISTs. DOG1.1 is also a sensitive marker for unusual GIST subgroups lacking KIT or PDGFRA mutations. In tumors that are negative for both KIT and DOG1.1, mutational screening may be required to confirm the diagnosis of GIST. Cellular Localization: Cell Membrane.
Formulation	State: Supernatant State: Tissue Culture Supernatant with 0.2% BSA and < 0.1% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	anoctamin 1
Database Link	Entrez Gene 3690 HumanEntrez Gene 55107 Human UniProt ID Q5XXA6
Background	DOG1 gene, a gastrointestinal stromal tumor (GIST) specific gene, encoding for the hypothetical protein FLJ10261, which was named Discovered on GIST 1 (DOG1). DOG1 protein is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGFR alpha mutation status.
Synonyms	Anoctamin-1, Transmembrane protein 16A, DOG-1, ORAOV2, TAOS2, TMEM16A
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton

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