Hepatocyte Specific Antigen (HSA) Mouse Monoclonal Antibody [Clone ID: OCH1E5]

Specifications

Product Data
Clone Name	OCH1E5
Applications	IF, IHC
Recommend Dilution	Immunofluorescence: 0.5-1 μg/ml. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: Liver or Hepatocellular Carcinoma (HCC).
Reactivity	Canine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Extract of a formalin-fixed, rejected-allograft of a Human liver.
Specificity	Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC’s occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. Clone OCH1E5 is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. OCH1E5 labels an antigen in the mitochondrial fraction of the liver homogenates. Cellular Localization: Finely granular cytoplasmic.
Formulation	10mM PBS State: Purified State: Liquid purified IgG fraction from Bioreactor Concentrate Stabilizer: 0.05% BSA Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Protein A/G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Background	Hepatoblastoma is the most common primary tumor of the liver in children. The use of specific hepatocyte markers and also of alpha Fetoprotein or carcinoembryonic antigen are useful for the identification of normal and malignant fetal hepatocytes.
Synonyms	HepPar-1, hepatocellular carcinoma marker, hepatocyte marker
Reference Data

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