Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: 35betaH11]

Specifications

Product Data
Clone Name	35betaH11
Applications	FC, IF, IHC, WB
Recommend Dilution	Western blotting.  Flow Cytometry. Immunocytochemistry.  Immunohistochemistry on Frozen Sections Recommended Dilutions: 1/100–1/500 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
Reactivity	Human, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal extract of a Human hepatocellular carcinoma cell line (Hep3B).
Specificity	35ßH11 reacts exclusively with Cytokeratin 8 which is present in glandular-type epithelia and most carcinomas derived thereof. It is in general not reactive in non-epithelial tissues and cells.Cytokeratin 8.  Cellular Localization: Cytoplasmic.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 8
Database Link	Entrez Gene 3856 Human UniProt ID P05787
Background	Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to distinguish between different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only Cytokeratin polypeptides 8 and 18.
Synonyms	KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A
Reference Data

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