NeuN (RBFOX3) Mouse Monoclonal Antibody [Clone ID: A60]

Specifications

Product Data
Clone Name	A60
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Brain tissue.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified cell nuclei from Mouse brain.
Specificity	NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. Cellular Localization: Nuclear.
Formulation	Purified antibody diluted in Tris HCI buffer containing stabilizing protein and <0.1% sodium azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	RNA binding protein, fox-1 homolog 3
Database Link	Entrez Gene 146713 Human UniProt ID A6NFN3
Background	NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
Synonyms	Fox-1 homolog C, FOX3
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.