S100A12 Mouse Monoclonal Antibody [Clone ID: 19F5]

Specifications

Product Data
Clone Name	19F5
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA: 1/2000-1/10000. Western Blot: 1/200-1/1000. Immunohistochemistry: 1/100-1/500.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant S100A12 protein
Specificity	Reacts with 10 kDa Human S100A12 protein. No cross reaction with the other S100 protein family.
Formulation	PBS State: Purified State: Lyophilized purified powder Preservative: None
Reconstitution Method	Restore in distilled water.
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	S100 calcium binding protein A12
Database Link	Entrez Gene 6283 Human UniProt ID P80511
Background	S100 family proteins are acidic low molecular weight proteins containing 2 EF-hand calcium binding domains. They are characterised by their cell-type specific expression patterns. S100A12 is a member of the calgranulin sub-family. Calgranulins are S100 proteins that are expressed by neutrophils and also found in infiltrating monocytes and granulocytes during chronic inflammation. S100A12 is expressed in neutrophils and monocytes. It is highly abundant in resting neutrophils, comprising 5% of total cytostolic protein. S100A12 is a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.
Synonyms	S100-A12, Calgranulin-C, CAGC, CGRP, CAAF1, EN-RAGE
Reference Data

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