beta III Tubulin (TUBB3) (tubulin beta-3 chain) Mouse Monoclonal Antibody [Clone ID: 3D9]

Specifications

Product Data
Clone Name	3D9
Applications	ELISA, IHC, IP, WB
Recommend Dilution	ELISA.  Western Blot: 1/500-1/1000.  Immunoprecipitation: 1/50-1/200. Immunohistochemistry: 1/100-1/500.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	beta-Tubulin protein.
Specificity	Reacts with Human 55 kDa TUBB3 protein. Cross reacts with Mouse, Rat and other species.
Formulation	PBS State: Purified State: Lyophilized purified antibody Preservative: None
Reconstitution Method	Restore in distilled water
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	tubulin beta 3 class III
Database Link	Entrez Gene 10381 Human UniProt ID Q13509
Background	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, α- and β-tubulin, are usually products of separate genes. The β-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III β-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III β-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.
Synonyms	Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4
Reference Data

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