Twist (TWIST1) Mouse Monoclonal Antibody [Clone ID: 3E10]

Specifications

Product Data
Clone Name	3E10
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1/1000-1/5000. Immunohistochemistry: 1/100-1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide derived from N-term domain of Human Twist-1 protein
Specificity	Reacts with Human 21 kDa TWIST1 protein. Cross reacts with Mouse, Rat and other species. It does not cross reacts with Twist-2.
Formulation	0.1M Tris, 0.1M Glycine and 2% Sucrose State: Purified State: Lyophilized powder Preservative: None
Reconstitution Method	Restore with 0.1 ml distilled water to receive a concentration of 1 mg/ml.
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	twist family bHLH transcription factor 1
Database Link	Entrez Gene 7291 Human UniProt ID Q15672
Background	Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
Synonyms	Twist-related protein 1, TWIST, BHLHA38
Reference Data

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