GFP Mouse Monoclonal Antibody [Clone ID: 168AT1211]

Specifications

Product Data
Clone Name	168AT1211
Applications	IF, IHC, WB
Recommend Dilution	Western blotting: 1/4,000. Immunohistochemistry: 1/50-1/100. Immunofluorescence: 1/50-1/100.
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	27 kDa
Database Link	UniProt ID P42212
Background	Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunoprecipitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
Synonyms	Green fluorescent protein, GFP-Tag
Reference Data

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