EGFR pTyr1016 Mouse Monoclonal Antibody [Clone ID: EM-12]

Specifications

Product Data
Clone Name	EM-12
Applications	FC, IP, WB
Recommend Dilution	Flow Cytometry. Immunoprecipitation. Western blot: 1 µg/ml. Positive control: EGF-stimulated A431. Negative control: Non-stimulated A431.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic phospho-peptide covering sequence around tyrosine 1016 of human EGFR
Specificity	The antibody EM-12 reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine 1016.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for up to one month or (in aliquots) at -20°C for longer.
Gene Name	epidermal growth factor receptor
Database Link	Entrez Gene 1956 Human UniProt ID P00533
Background	The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
Synonyms	Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
Reference Data
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
Protein Pathways	Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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