KDEL Receptor (KDELR1) (C-term) Mouse Monoclonal Antibody [Clone ID: 2E7]

Specifications

Product Data
Clone Name	2E7
Applications	IF, WB
Recommend Dilution	Western blot: 1/1000-1/2000. 1 µg/ml of this antibody was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG -HRP as the secondary antibody. Immunofluorescence: 1/50-1/500.
Reactivity	Drosophila, Plant, Yeast
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic HDEL peptide corresponding to the C-terminus of Yeast Bip.
Specificity	Detects ~78kDa protein. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in Yeast, Plant, nematode and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
Formulation	PBS, pH 7.4 containing 50% Glycerol with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	KDEL endoplasmic reticulum protein retention receptor 1
Database Link	Entrez Gene 10945 Human UniProt ID P24390
Background	HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3).
Synonyms	KDEL endoplasmic reticulum protein retention receptor 1, ERD2.1, ER marker
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Vibrio cholerae infection

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