Aurora A (AURKA) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F12]

Specifications

Product Data
Clone Name	7F12
Applications	WB
Recommend Dilution	Immunobotting (Western Blot): 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blot incubation buffer. Included Postitive Control: Cell Lysate from untreated A431 cells.
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin Epitope: N-terminus.
Specificity	Recognizes Aurora-A (N-term).
Formulation	PBS / 0.09% Sodium Azide / PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name	aurora kinase A
Database Link	Entrez Gene 20878 MouseEntrez Gene 261730 RatEntrez Gene 6790 Human UniProt ID O14965
Background	Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2; STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.
Synonyms	AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2
Note	Molecular Weight: 47 kDa Protocol: Positive Control: Cell Lysate from untreated A431 cells. Formulation: Lyophilized Cell lysate from Serum starved A431 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of Positive Control correspond to ca. 20.000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Oocyte meiosis

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