8-Hydroxy-Guanosine / 8-OHG Mouse Monoclonal Antibody [Clone ID: N45.1]

Specifications

Product Data
Clone Name	N45.1
Applications	ELISA, IF, IHC
Recommend Dilution	ELISA (Ref.3). Immunohistochemistry on Paraffin Sections: 3-5 µg/ml (See Ref.1-6 for more details).  Immunocytochemistry (Ref.7).
Reactivity	Human, Mouse, Rabbit, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	8-OHdG conjugated Keyhole Limpet Hemocyanin
Specificity	Recognizes 8-OHdG. 19 analogues of 8-OHdG {guanosine (G), 7-methyl-G, 6-SH-G, 8-bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG, 8-OHdA, guanine (Gua), O6-methyl-Gua, 8-OHGua, uric acid, Urea, creatine, creatinine} demonstrate no cross-reactivity. Only 8-sulfhydryl-G and 8-OHG demonstrate minimal cross-reactivity (less than 1%). Monoclonal antibody (clone N45.1) is highly specific for DNA damage, not cross react with RNA oxidation products such as 8-hydroxy-guanine and 8-hydroxy-guanosine.
Formulation	State: Purified. 10 mM Phosphate buffered saline, pH7.4, 1.0% BSA State: Lyophilized.
Reconstitution Method	Resuspend in 200 µl aqua bidest to 0.1 mg/ml
Purification	Ammnonium Sulphate Precipitation
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	8 Hydroxyguanosine (80G) is a modified base which occurs in DNA as a result of oxidative stress. 8 Hydroxy-2'deoxyguanosine (8OHdG) has become a sensitive marker of oxidative damage in cellular DNA and has been reported to be excreted in the urine. Levels of 8OHdG have been shown to increase on exposure to ionizing radiation and tobacco smoke.
Synonyms	8-Hydroxy-2'-deoxyguanosine, 8-Hydroxyguanine, 8-Hydroxyguanosine, 8-OFdG, 8-OHG, 8OG, 8OHG, 8OHdG
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.