Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody [Clone ID: CK-18]

Specifications

Product Data
Clone Name	CK-18
Applications	IHC, WB
Recommend Dilution	Western blot: Use at 0.5-1 μg/ml with the appropriate system to detect Cytokeratin peptide 18 in cells and tissues. Immunohistochemistry on Frozen Sections: Use at 0.5-2 μg/ml to detect Cytokeratin peptide 18 in Formalin or Acetone Fixed Tissues. Immunohistochemistry on Paraffin Sections: Use at 1-2 μg/ml to detect Cytokeratin peptide 18 in Formalin Fixed and Paraffin Embedded Tissues.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human epidermal carcinoma A-431 and MCF-7 Human breast cancer cell lines.
Specificity	Recognizes Cytokeratin Peptide 18. No cross reactivity with other proteins.
Formulation	1.2% Sodium Acetate containing 2mg BSA and 0.01 mg Sodium Azide as preservative State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1.2% Sodium Acetate or neutral PBS.
Concentration	0.1 mg/ml (after reconstitution with 1 ml PBS).
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody after reconstitution at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 18
Database Link	Entrez Gene 294853 RatEntrez Gene 3875 Human UniProt ID P05783
Background	Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18(KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis
Synonyms	Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18
Reference Data
Protein Pathways	Pathogenic Escherichia coli infection

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