HSP27 (HSPB1) Mouse Monoclonal Antibody [Clone ID: SJ-25]

Specifications

Product Data
Clone Name	SJ-25
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 0.5 - 2 μg/ml. Immunohistochemistry on frozen and paraffin sections: 1 - 2 μg/ml. Immunocytochemistry.
Reactivity	Human, Turkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle
Specificity	This antibody reacts to HSPB1 / HSP27.
Formulation	1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative. State: Purified State: Lyphilized purified Ig fraction
Reconstitution Method	Restore with 1.2% sodium acetate or neutral PBS
Concentration	0,1 mg/ml (after reconstitution with PBS)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	heat shock protein family B (small) member 1
Database Link	Entrez Gene 3315 Human UniProt ID P04792
Background	The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
Synonyms	Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25
Reference Data
Protein Pathways	MAPK signaling pathway, VEGF signaling pathway

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