S100B Mouse Monoclonal Antibody [Clone ID: SA-12]

Specifications

Product Data
Clone Name	SA-12
Applications	IF, IHC, IP, WB
Recommend Dilution	Western Blot: Use at 2 µg/ml with the appropriate system to detect S-100b in cells and tissues. Immunohistochemistry on Paraffin Sections: Use at 1-2 μg/ml to detect S-100b in formalin fixed and paraffin embedded tissues.
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Bovine brain S-100b.
Specificity	This antibody recognizes S100B. No cross reactivity with other proteins.
Formulation	1.2% Sodium Acetate with 2 mg BSA and 0.01 mg Sodium Azide as preservative. State: Purified State: Lyphilized purified Ig fraction
Reconstitution Method	Restore with 1.2% Sodium Acetate or neutral PBS
Concentration	0.1 mg/ml (after reconstitution with 1 ml PBS)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 525716 Bovine UniProt ID P02638
Background	S100 calcium binding protein B or S100beta is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery.1 S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al. (1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.
Synonyms	Protein S100-B, S100 beta, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, Astrocyte Marker
Reference Data

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