6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: HIS.H8]

Specifications

Product Data
Clone Name	HIS.H8
Applications	IF, IP, WB
Recommend Dilution	Western blot: 1/1000-1/4000. Immunoprecipitation: 1/200-1/400. Immunostaining: 1/200-1/400.
Reactivity	Mammalian
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide (KLH-coupled) containing the His epitope 6x His (H-H-H-H-H-H).
Specificity	His-tag monoclonal antibody recognizes His-tagged tagged proteins overexpressed in cells, including both amino-or carboxy-termini of targeted proteins in transfected mammalian cells. Reacts with Human, Mouse, Rat and Others.
Formulation	10mM PBS, pH 7.2 State: Aff - Purified State: Liquid purified IgG fraction Preservative: 0.05% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni++-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.
Synonyms	6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag
Reference Data

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