DYKDDDDK Epitope Tag Mouse Monoclonal Antibody [Clone ID: G191]

Specifications

Product Data
Clone Name	G191
Applications	IF, IP, WB
Recommend Dilution	Western Blot: 1/1000-1/4000. Immunoprecipitation : 1/200. Immunofluorescence : 1/100.
Reactivity	All Species
Host	Mouse
Clonality	Monoclonal
Immunogen	A synthetic peptide (DYKDDDDK) coupled to KLH.
Specificity	This antibody Recognizes over-expressed proteins containing DYKDDDDK epitope tag fused to either amino-or carboxy-termini of targeted proteins in transfected mammalian cells.
Formulation	PBS (pH 7.2) with 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
Synonyms	FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag
Reference Data

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