Il2ra Rat Monoclonal Antibody [Clone ID: PC61.5.3]

Specifications

Product Data
Clone Name	PC61.5.3
Applications	FC
Recommend Dilution	Immunoprecipitation. Flow Cytometry.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	B6.1 CTL Cell line Donor: OFA rat spleen Fusion Partner: mouse myeloma line P3X63Ag8.653
Specificity	This monoclonal antibody reacts with the low affinity alpha chain of the Interleukin-2 receptor antigen present on activated T and B cells in mice. AM26037FC inhibits IL-2 binding and IL-2 dependent proliferation.
Formulation	PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: FITC State: Liquid purified Ig fraction Label: Fluorescein isothiocyanate isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.  This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	interleukin 2 receptor, alpha chain
Database Link	Entrez Gene 16184 Mouse UniProt ID P01590
Synonyms	Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0.5-1.0 μg of AM26037FC per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.) 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/c Cell Concentration: 1x10e6 cells per tests Antibody Concentration Used: 1.0 μg/10e6 cells Isotypic Control: FITC Rat IgG1 Cell Source Percentage of cells stained above control: T Cell Blasts (Con A activated) 90.9% Thymus (unactivated) 3.8%
Reference Data

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