CD62E (SELE) Rat Monoclonal Antibody [Clone ID: 4D10]

Specifications

Product Data
Clone Name	4D10
Applications	FC
Recommend Dilution	Has been reported to work in FACS. Suggested positive control: Human endothelial cells in acutely inflamed tonsil.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	LPS-stimulated human endothelial cells
Specificity	This antibody detects CD62E / E-Selectin.
Formulation	Phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.09% sodium azide as a preservative Label: FITC State: Liquid Ig fraction
Concentration	0.5 mg/ml
Purification	Affinity chromatography
Conjugation	FITC
Storage Condition	Store the antibody at 2-8 °C.
Gene Name	selectin E
Database Link	Entrez Gene 6401 Human UniProt ID P16581
Background	CD62E belongs to the selectin family and is thought to mediate rolling of myeloid cells and a subset of memory T cells on activated endothelium. The antigen is a 589 amino acid transmembrane protein. It consists of a C-type lectin-like domain, an epidermal growth factor (EGF)-like domain, six short consensus repeat (SCR) domains, a transmembrane domain and a cytoplasmic tail. CD62E is a counter-receptor for sialyl Lewis X, sialyl Lewis a and CLA (cutaneous lymphocyte antigen). It is involved in adhesion between myeloid cells and activated endothelium, and supports leucocytes rolling. Some skin-associated memory T cells express CLA, and it may be that CD62E is also involved in their homing to cutaneous sites of chronic inflammation. In addition, CD62E may also be involved in tumour metastasis. A soluble form of CD62E has been reported to mediate angiogenesis in pathological disorders.
Synonyms	SELE, ELAM1, LECAM2
Note	Antigen distribution: Isolated cells: Positive on LPS, TNFα, IL-1, or TPA stimulated endothelial cells (HUVECs) in tissue culture. No cross reactivity with other cell types known. Tissue sections: Strongly positive on human endothelial cells in acutely inflamed tissues such as atopic dermatitis or contact dermatitis 24h after sensitization; in the healthy spleen and lymph node. Few single cells express the 4D10 antigen. The antigen is generally absent in chronically inflamed tissues.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs)

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