BPV-1 E2 tag (SSTSSDFRDR) Mouse Monoclonal Antibody [Clone ID: 5E11]

Specifications

Product Data
Clone Name	5E11
Applications	IF, IP, WB
Recommend Dilution	Western blot (1/1000–1/5000): Dilute to a concentration of 1.0-0.2 µg/ml in a diluent containing 2% non-fat dry milk in TBST (Tris-Buffered Saline, Tween-20) (Background can be reduced by adding NaCl up to a concentration of 1.5 M). Incubate the membrane with diluted anti-E2Tag antibody for 1 hour at room temperature with gentle orbital shaking. Immunofluorescence (1/1000): Dilute anti-E2Tag antibody to a concentration of 1-2 µg/ml in a diluent containing 1% BSA in PBS. Incubate with diluted anti-E2Tag antibody for 1 hour at room temperature. Immunoprecipitation. This antibody belongs to the IgG1 subclass, thus, we recommend using protein G-Agarose for Binding experiments.
Reactivity	Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant full length BPV type 1 E2 protein purified from E. coli
Specificity	This antibody detects Bovine Papillomavirus E2-protein mapped to aa 199-208 (E2Tag, SSTSSDFRDR). Isolates E2 Tag-tagged proteins and associated proteins/protein binding partners by Immunoprecipitation.
Formulation	PBS with 50% Glycerol State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Upon receipt, store (in aliquots) at -20°C to -70 °C. Avoid repeated freezing and thawing.
Database Link	UniProt ID P03122
Background	The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
Synonyms	BPV1, Bovine Papilloma Virus Protein E2
Reference Data

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