BPV-1 Protein E2 (182-190) Mouse Monoclonal Antibody [Clone ID: 1E2]

Specifications

Product Data
Clone Name	1E2
Applications	IF, IP, WB
Recommend Dilution	Western blot: 1/500 – 1/2000; dilute to a concentration of 4.0-0.5 µg/ml in a diluent containing 2% non-fat dry milk in TBST (Tris-Buffered Saline, Tween-20). Incubate the membrane with diluted anti-E4Tag antibody for 1 hour at room temperature with gentle orbital shaking. Immunofluorescence: 1/1000; dilute anti-E4Tag antibody to a concentration of 1-2 µg/ml in a diluent containing 1% BSA in PBS. Incubate with diluted anti-E4Tag antibody for 1 hour at room temperature. Immunoprecipitation: Isolates E4Tag-tagged proteins and associated proteins/protein binding partners. This antibody belongs to the IgG1 subclass, thus, we recommend using protein G-agarose for binding experiments. ELISA.
Reactivity	Bovine
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody detects E4Tag and Bovine Papillomavirus E2- protein, mapped to amino acids 182-190 (TTGHYSVRD).
Formulation	PBS State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 50% glycerol
Concentration	lot specific
Purification	Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Database Link	UniProt ID P03122
Background	The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
Synonyms	BPV1 Regulatory protein E2
Reference Data

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