stxA2 Mouse Monoclonal Antibody [Clone ID: 11E10]

Specifications

Product Data
Clone Name	11E10
Applications	ELISA, FN, WB
Recommend Dilution	Western blot: 1:10 as starting dilution. Inhibition of the biological activity of the toxin.
Reactivity	Escherichia coli
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody recognizes the 2A subunit of Shiga-like toxin 2.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Product should be stored at 2-8 °C.
Database Link	UniProt ID P09385
Background	Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11, and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. Antibodies to SLT-II can also neutralize a variant of SLT-II (designated SLT-Iiv) produced by strains of E.coli that cause edema. SLT-IIv is cytotoxic for Vero but not HeLa cells, distinguishing it from SLT-II. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
Synonyms	SLT-2 A subunit, SLT-2a, SLT-IIa, stx2A, stxA2, Verotoxin 2 subunit A, Verocytotoxin 2 subunit A
Reference Data

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