CD73 (NT5E) Mouse Monoclonal Antibody [Clone ID: 4G4]

Specifications

Product Data
Clone Name	4G4
Applications	FC, FN, IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections (1/50 as starting dilution): Tissue sections were fixed in acetone. As Positive Control anti-CD31 was used and as Negative Control an isotype antibody (Ref.5). Flow Cytometry (1/50 as starting dilution): Antibody 4G4 stains the extracellular domain of CD73. As Positive Control anti-CD3 was used and as Negative Control an irrelevant antibody (Ref.1). Functional Assay: Antibody 4G4 functions as an inhibitor of lymphocyte binding to HUVEC. The antibody was functionally tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer (Ref.1). Furthermore the monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes (Ref.5) and reduces enzyme activity (Ref.2). Immunofluorescence: HUVEC cells were seeded on gelatin-coated coverslips and stained with antibody (Ref.5). Immunoprecipitation (Ref.1-3). Western blot (Ref.3). Positive Control: HUVEC and fresh lymphocytes. Negative Control: COS cells (Ref.2).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Inflamed synovial stroma from rheumatoid arthitis patients.
Specificity	This antibody detects CD73. The monoclonal antibody 4G4 recognizes both membrane bound and soluble Human CD73, also known as ecto-5'-Nucleotidase.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	5'-nucleotidase ecto
Database Link	Entrez Gene 4907 Human UniProt ID P21589
Background	CD73 is a 70-kDa GPI-anchored cell surface molecule and belongs to the 5’-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5’-nucleotidase activity is an important mediator of the anti-inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo. In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency.
Synonyms	NT5, NTE, NT5E
Reference Data

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