VAP1 (AOC3) Mouse Monoclonal Antibody [Clone ID: 174-5]

Specifications

Product Data
Clone Name	174-5
Applications	FN, IF, IHC
Recommend Dilution	Immunohistochemistry on frozen sections: Tissue sections were fixed in acetone before staining with 174-5. As positive control anti-VAP-1 mAb 2D10 was used and as negative control an isotype matched irrelevant mAb. (Ref.1). Typical starting working dilution is 1:50. Flow cytometry: Stains Ax cells stably transfected with VAP-1 cDNA.. As negative control mock transfected cells were used.(Ref.1). Typical starting working dilution is 1:50. Functional assay: Inhibits lymphocyte infiltration in liver allograft rejection. The antibody was administered intravenously at a concentration of 2 mg/kg. A irrelevant isotype-matched antibody served as a negative control. (Ref.1). Immunoflourescence (Ref1,2). Positive control: Ax cells stably transfected with VAP-1 cDNA. (Ref. 1). Negative control: Mock transfected Ax cells. (Ref. 1).
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified vessels from human peripheral lymph nodes (ref 1)
Specificity	This antibody detects Vascular Adhesion Protein-1 (VAP-1).
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	amine oxidase, copper containing 3
Database Link	Entrez Gene 8639 Human UniProt ID Q16853
Background	Vascular Adhesion Protein-1 (VAP-1) is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity. Elevated sVAP-1serum levels have been described in several inflammatory diseases as well as colorectal cancer. Moreover, diminished insulin secretion appears to increase the concentration of soluble VAP-1 in plasma. Therefore, VAP-1 might be an interesting diagnostic marker as well therapeutic target for modulating inflammation.
Synonyms	HPAO
Reference Data

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