MARCO (420-431) Mouse Monoclonal Antibody [Clone ID: PLK-1]

Specifications

Product Data
Clone Name	PLK-1
Applications	FC, FN, IF, IHC, IP, WB
Recommend Dilution	Immunohistochmistry on Frozen Sections: Tissue sections can be fixed in acetone or 2% paraformaldehyde. PLK-1 antibody was used at 5 µg/ml (Ref.3,4). Flow Cytometry: Antibody PLK-1 weakly stains alveolar macrophages by recognizing the extracellular domain of MARCO. Transfected COS cells were used as positive control (Ref.1). Functional assay: Antibody PLK-1 blocks human alveolar macrophages binding to unopsonized particles (Ref.1,3,5). Immunofluorescence: Aveolar macrophages and transfected CHO cells were stained for MARCO using 0.6 µg/ml PLK-1 antibody (Ref.2,5). Immunoprecipitation: Antibody PLK-1 immunoprecipitates MARCO as 60 and 50 kDa protein from lysates obtained from COS cells transfected with human MARCO (Ref.3). Western blot: Antibody PLK-1 stained MARCO under non-reducing conditions (Ref.6). Positive Control: Human alveolar macrophages. Negative Control: All other Human cells.
Reactivity	Bovine, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human alveolar macrophages
Specificity	This antibody binds specifically to MARCO (recognizes domain V between residues 420 and 431), and has been shown to partially block ligand binding.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	macrophage receptor with collagenous structure
Database Link	Entrez Gene 8685 Human UniProt ID Q9UEW3
Background	The scavenger receptors (SRs) expressed by macrophages are thought to play an important role in the immune response against bacteria by mediating ligand binding and phagocytosis. SRs can be divided into three different classes based upon their structural properties, which are termed SR-A, SR-B and SR-C. SRs-A are homotrimeric glycoproteins composed of 77 kDa monomers subdivided into 3 types.. The molecular structure of MARCO resembles that of SR-A type I, containing a triple-helical collagenous domain and a scavenger receptor cysteine-rich (SRCR) domain at the C terminus. MARCO is only expressed in some subpopulations of macrophages, although it’s expression can be strongly upregulated during infection or LPS treatment. Furthermore, MARCO is, like sinusoidal endothelial cell markers DC-SIGNR, LYVE-1 and stabilin-2, expressed by sinusoidal endothelial cells in lymph node. MARCO expressed by alveolar macrophages seems to play an important role in response to inhaled particles and airborne pathogens.
Synonyms	macrophage receptor with collagenous structure, Scavenger Receptor
Reference Data

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