CD36 Mouse Monoclonal Antibody [Clone ID: FA6-152]

Specifications

Product Data
Clone Name	FA6-152
Applications	ELISA, FN, IF, IHC, WB
Recommend Dilution	Immnohistochemistry on frozen sections (4,5): Tissue embedded in tissue-tek (for instance aortic tissue) followed by freezing in liquid nitrogen; 7-8 µm sections; air-dried; aceton-fixed; 10 % NGS as block (Ref 4). The typical starting working dilution is 1:50. Flow cytometry (1): stains the extracellular domain of CD36. Unfixed cells; 2µg per 100.000 cells. Positive on granulocytes (Ref 1). The typical starting working dilution is 1:50. Functional studies (2): Platelet aggregation and secretion was induced by > 1µg/ml antibody (Ref 2). Immunoassay (3): 10 µg/ml antibody as coat diluted in Tris-buffered saline ; 100 µl/well; o/n at RT (Ref 3). Immunoflourescence (1,4): Unfixed cells were incubated for 30 minutes at 4 °C followed by a secondary FITC polyclonal antibody; one-minute methanol fixation before analysis (Ref 1). Immunoprecipitation: 88 kDa sialoglycoprotein in platelets; 85 and 88 kDa in HEL cells. 10 µg antibody/200 µg protein. Positive control: HELA cells.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	20-Weeks-old fetal erythrocytes
Specificity	This antibody recognizes human CD36 (88-kDa), a cell surface class B scavenger receptor, also known as thrombospondin receptor. It blocks the biological activity of CD36 by blocking collagen/thrombospondin binding. The antibody agglutinates fetal but not adult erythrocytes.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	CD36 molecule
Database Link	Entrez Gene 948 Human UniProt ID P16671
Background	CD36 is a heavily N-glycosylated transmembrane protein of ~88 kDa with two short intracellular domains and a large extracellular domain. The protein is sensitive for neuroaminidase, resulting in a shift from 88 to 85 kDa. CD36 is expressed on platelets, mature monocytes and macrophages, microvascular endothelial cells, mammary endothelial cells, during stages of erythroid cell development and on some macrophage derived dendritic cells. The antibody recognizes adult and fetal monocytes, platelets and reticulocytes, but doesn’t stain lymphocytes and granulocytes. Reactivity has also been found in small intestine, kidney, liver and thyroid. CD36 expression is primarily controlled by the transcription heterodimer PPARg-RXR (peroxisome proliferator-activated receptor-g-retinoid-X-receptor). CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adaptor molecules. It is a receptor and transporter of oxidized lipids and long chain fatty acids. CD36 has been implicated in many biological processes including angiogenesis, phagocytosis, inflammation, and lipid and glucose metabolism. Several in vivo models support the role of the thrombospondin / CD36 system in angiogenesis and tumor growth. An important role for CD36 has been found in Malaria as major receptor for P. falciparum-infected red blood cells. CD36 is associated with Src-family kinases and with the integrins α3β1 and α6β1. Recently, CD36 has been identified as a protein that is required for toll like receptor (TLR2) recognition of di-acylated bacterial lipopeptides and lipoteichoic acid4. Furthermore, CD36 has been shown to function as phagocytic receptor for apoptotic cells. Many different ligands have been reported to interact with CD36, suggesting that CD36 could recognize a structure-based domain rather than specific contact residues.
Synonyms	Glycoprotein IIIb, PAS IV, PAS-4, Thrombospondin receptor, GP3B, GP4
Note	The endotoxin concentration is < 24 EU/mg, determined with HIT302 LAL Assay.
Reference Data

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